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KMID : 0380620200520060580
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Korean Journal of Food Science and Technology
2020 Volume.52 No. 6 p.580 ~ p.586
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Validation of a trienzyme-Lactobacillus casei method for folate analysis in fishery resources consumed in the Korean diet
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Jeong Bo-Mi
Nam Ki-Ho
Kim Yeon-Kye
Chun Ji-Yeon
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Abstract
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Fishery resources have been widely consumed as protein- and vitamin-rich food sources in the Korean diet.
However, information regarding their vitamin levels is extremely limited. In this study, trienzyme-Lactobacillus casei method was validated and used to determine the folate contents in fishery foods. The trienzyme-L. casei method for folate analysis showed excellent accuracy (85.2 to 95.3% recovery) and precision (repeatability 1.4% RSD and reproducibility 2.4% RSD). Folate contents of 20 fish foods (4 fish, 3 crustaceans, 3 sea algae, 3 cephalopods, 4 shellfish, and 3 others) ranged from 1.75 to 97.98 μg/100 g. Furthermore, we found that the folate content in seaweed fusiforme was the highest, followed by gulfweed (69.73 μg/100 g). Folate analysis using the trienzyme-L. casei method was determined excellent based on the z-score of ?0.3 in the Food Analysis Performance Assessment Scheme test. Analytical and method validation data generated in this study could be used to update the national food composition table on vitamin B9 in Korean fishery resources.
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KEYWORD
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fishery resources, folate, validation, database, vitamin
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